A new subfamily classification of the <i>Citrus</i> family (Rutaceae) based on six nuclear and plastid markers

Rutaceae is a family of angiosperms well known for the economically important genus Citrus. The division into subfamilies still inadequate and provisional. Previous phylogenetic studies at level are characterized by limited sampling genera lack several crucial taxa. Here, we present study based on six nuclear plastid markers including 87.7% currently accepted genera, which more than twice as many in previous studies. Seven included analysis first time. Most clades resolved with high support, propose new subfamily classification that comprises Amyridoideae, Aurantioideae, Cneoroideae, Haplophylloideae, Rutoideae Zanthoxyloideae. Aurantioideae only traditional monophyletic. We tested whether 13 morphological karyological characters taxonomically informative Rutaceae. Chromosome numbers clearly different two main Rutaceae, but fruit characteristics, have been used to define past, do not distinguish between lineages family.